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Apostle 技术在心血管疾病研究领域的应用案例

Apostle 技术在许多国际领先的科研、临床实验室、公共卫生监测领域广泛应用。下面是一些在心血管疾病应用的案例。对于更多引用 Apostle 技术的文献、客户评价和反馈,请访问这里

Apostle MiniMax 技术在心血管疾病临床研究中的应用

Electronic Cigarettes Induce Mitochondrial DNA Damage and Trigger TLR9 (Toll-Like Receptor 9)-Mediated Atherosclerosis. 

Li et al.  Arteriosclerosis, Thrombosis, and Vascular Biology. 2021;41:839–853


Electronic cigarette (e-cig) use has recently been implicated in promoting atherosclerosis. In this study, we aimed to investigate the mechanism of e-cig exposure accelerated atherosclerotic lesion development.

Approach and Results:

Eight-week-old ApoE−/− mice fed normal laboratory diet were exposed to e-cig vapor (ECV) for 2 hours/day, 5 days/week for 16 weeks. We found that ECV exposure significantly induced atherosclerotic lesions as examined by Oil Red O staining and greatly upregulated TLR9 (toll-like receptor 9) expression in classical monocytes and in the atherosclerotic plaques, which the latter was corroborated by enhanced TLR9 expression in human femoral artery atherosclerotic plaques from e-cig smokers. Intriguingly, we found a significant increase of oxidative mitochondria DNA lesion in the plasma of ECV-exposed mice. Administration of TLR9 antagonist before ECV exposure not only alleviated atherosclerosis and the upregulation of TLR9 in plaques but also attenuated the increase of plasma levels of inflammatory cytokines, reduced the plaque accumulation of lipid and macrophages, and decreased the frequency of blood CCR2+ (C-C chemokine receptor type 2) classical monocytes. Surprisingly, we found that cytoplasmic mitochondrial DNA isolated from ECV extract-treated macrophages can enhance TLR9 activation in reporter cells and the induction of inflammatory cytokine could be suppressed by TLR9 inhibitor in macrophages.


E-cig increases level of damaged mitochondrial DNA in circulating blood and induces the expression of TLR9, which elevate the expression of proinflammatory cytokines in monocyte/macrophage and consequently lead to atherosclerosis. Our results raise the possibility that intervention of TLR9 activation is a potential pharmacological target of ECV-related inflammation and cardiovascular diseases.

Mice plasma was first centrifuged at 16 000×g for 10 minutes at 4 °C, and the supernatant was subjected to cell-free DNA (cfDNA) extraction using Apostle minimax cfDNA extraction kit (ApostleBio, CA). 

对于更多应用和引用 Apostle 技术的文献、客户评价和反馈,请访问这里

在 Apostle MiniMax 高效游离核酸分离技术的协助下,近期一项临床研究证实循环游离DNA在肝细胞肝癌检测中的临床价值,展现出高敏感度、特异度。此项研究发表在 Science Translational Medicine.