Apostle MiniMax 高效游离RNA 分离试剂盒

特别设计用于游离RNA cfRNA 分离

Overview, Apostle MiniMax cfRNA

cfRNAs in biofluids like serum or plasma usually presents as short fragments<1000 nt, and even smaller cell-free miRNA ~20 nt. Although the concentration of cfRNAs is extremely low, cfRNAs are crucial biomarkers for cancer and other diseases. Powered by Apostle MiniMax technology, this cfRNA isolation kit is featured for its efficient recovery of all RNAs in the range between 17-1000 nt, without using phenol or chloroform, and ready for a broad range of subsequent applications, including sequencing, PCR, etc.

客户评价、引用、报道和反馈

【Nature Communications】Terminal modifications independent cell-free RNA sequencing enables sensitive early cancer detection and classification
"We perform transcriptome-wide characterizations in 165 lung cancer, 30 breast cancer, 37 colorectal cancer, 55 gastric cancer, 15 liver cancer, and 133 cancer-free participants and demonstrate its ability to identify transcriptomic changes occurring in early-stage tumors. "..."(Methods section) 200 μl of plasma samples were subjected to cfRNA extraction using the Apostle MiniMax High-Efficiency cfRNA Isolation Kit (Apostle), following the manufacturer’s protocol with minor modifications. "

- 发表于 Nature Communications 的独立临床研究

Combining cell-free RNA with cell-free DNA in liquid biopsy for hematologic and solid tumors
"This data suggests that liquid biopsy combining analysis of cfRNA with cfDNA is practical and may provide helpful information in predicting genomic abnormalities, diagnosis of neoplasms and evaluating both the tumor biology and the host response. "..."(Methods section) We used Apostle MiniMax High Efficiency cfRNA/cfDNA isolation kit and followed the recommended protocol. After extraction, half of the cfDNA was treated with DNase to obtain cfRNA and the other half was used for DNA studies. "

- 发表于 Heliyon (Cell Press) 的独立临床研究

Performance Data

Apostle MiniMax provides superior RNA isolation efficiency for RNA ladders spiked in biological medium. The isolation efficiency over 90% can be achieved, for both small RNAs (17-150 nt) and RNAs up to 1000nt (Exhibit 1).

Apostle MiniMax High Efficiency cfRNA Isolation Kit is suitable for processing plasma collected in various major blood collection tubes (BCTs). Compared to other competitors (a major column-based product), Apostle MiniMax TM High Efficiency cfRNA Isolation Kit provides consistently better performance (1.1-2000 times higher, Exhibit 2).

The conclusion is further confirmed by endogenous mRNA and miRNAs isolation and qPCR measurements. cfRNA was isolated from 200 uL plasma, using Apostle MiniMax High Efficiency cfRNA Isolation Kit, and a major column based competitor. Blood were collected in K3EDTA BCTs or major specialized cfRNA BCTs and processed to obtain cell-free plasma. Beta-globin, miR-21, miR-U6, and miR-15a were measured by qPCR using the isolated cfRNA, where Apostle MiniMaxTM High Efficiency cfRNA Isolation Kit provides 1.1-480 times higher isolation efficiency compared to major competitor (Exhibit 3-5).

In summary, Apostle MiniMax High Efficiency cfRNA Isolation Kit offers superior isolation efficiency of cell-free RNAs between 17 nt to 1000 nt, without phenol or chloroform. It is suitable for processing samples collected in various major blood collection tubes, especially specialized cfRNA BCTs which will prevent RNA degradation during storage.

Exhibit 1. Over 90% RNA recovery in range between 17 – 1000 nt.

RNA (100-1000 nt) and small RNA ladder (17-150 nt) was spiked in serum or plasma, followed by isolation with Apostle MiniMax High Efficiency cfRNA Isolation Kit (red curve), and compared with original RNA ladder (blue curve) using Bioanalyzer 2100 (A) RNA pico kit for 100-1000 nt and (B) small RNA kit for 17-150 nt fragments. Apostle MiniMax High Efficiency cfRNA Isolation Kit offers superior RNA and small RNA recovery efficiency of > 90%.

Exhibit 2. 1.1 to 2000 times higher exogenous RNA recovery rate than a column-based major alternative product.

cfRNA was isolated from plasma using Apostle MiniMax TM High Efficiency cfRNA Isolation Kit (red) or a column-based major competitor (green). A synthetic RNA mimic cel-miR-39 was spiked in during the extraction procedure as the reference to evaluate recovery rate. The qPCR amplification plot and CT value show 1.1, 9, and 2000 times higher RNA recovery rate when compared to column-based major alternative product for plasma collected in K3EDTA tube, cfRNA BCT vendor 1, or cfRNA BCT vendor 2, respectively.

Exhibit 3. 1.1 to 2.4 times higher RNA recovery rate than a column-based major alternative product.

cfRNA was isolated from plasma (collected in K3EDTA BCT) using Apostle MiniMax High Efficiency cfRNA Isolation Kit (red) or a major alternative product (green). RNA and small RNA targets including beta-globin, miR-21, U6, and miR-15 were quantified by qPCR amplification plots and CT values. The cfRNA amount isolated using Apostle MiniMax High Efficiency cfRNA Isolation Kit are 1.1 to 2.4 times higher than the column-based alternative product.

Exhibit 4. 3.5 to 480 times higher RNA recovery rate than a column-based major alternative product.

cfRNA was isolated from plasma (collected in cfRNA BCT vendor 1) using Apostle MiniMax High Efficiency cfRNA Isolation Kit (red) or a major alternative product (green). RNA and small RNA targets including beta-globin, miR-21, U6, and miR-15 were quantified by qPCR amplification plots and CT values. The amount of cfRNA isolated using Apostle MiniMax High Efficiency cfRNA Isolation Kit are 3.5 to 480 times higher than the column-based alternative product.

Exhibit 5. 1.6 to 6 times higher RNA recovery rate than a column-based major alternative product.

cfRNA was isolated from plasma (collected in cfRNA BCT vendor 2) using Apostle MiniMax High Efficiency cfRNA Isolation Kit (red) or a major alternative product (green). RNA and small RNA targets including beta-globin, miR-21, U6, and miR-15 were quantified by qPCR amplification plots and CT values. The amount of cfRNA isolated using Apostle MiniMax High Efficiency cfRNA Isolation Kit are 1.6 to 6 times higher than the column-based alternative product.