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Apostle MiniMax 高效游离RNA 分离试剂盒

特别设计用于游离RNA cfRNA 分离

Overview, Apostle MiniMax cfRNA

cfRNAs in biofluids like serum or plasma usually presents as short fragments<1000 nt, and even smaller cell-free miRNA ~20 nt. Although the concentration of cfRNAs is extremely low, cfRNAs are crucial biomarkers for cancer and other diseases. Powered by Apostle MiniMax technology, this cfRNA isolation kit is featured for its efficient recovery of all RNAs in the range between 17-1000 nt, without using phenol or chloroform, and ready for a broad range of subsequent applications, including sequencing, PCR, etc.

Performance Data

Apostle MiniMax provides superior RNA isolation efficiency for RNA ladders spiked in biological medium. The isolation efficiency over 90% can be achieved, for both small RNAs (17-150 nt) and RNAs up to 1000nt (Exhibit 1).

Apostle MiniMax High Efficiency cfRNA Isolation Kit is suitable for processing plasma collected in various major blood collection tubes (BCTs). Compared to other competitors (a major column-based product), Apostle MiniMax TM High Efficiency cfRNA Isolation Kit provides consistently better performance (1.1-2000 times higher, Exhibit 2).

The conclusion is further confirmed by endogenous mRNA and miRNAs isolation and qPCR measurements. cfRNA was isolated from 200 uL plasma, using Apostle MiniMax High Efficiency cfRNA Isolation Kit, and a major column based competitor. Blood were collected in K3EDTA BCTs or major specialized cfRNA BCTs and processed to obtain cell-free plasma. Beta-globin, miR-21, miR-U6, and miR-15a were measured by qPCR using the isolated cfRNA, where Apostle MiniMaxTM High Efficiency cfRNA Isolation Kit provides 1.1-480 times higher isolation efficiency compared to major competitor (Exhibit 3-5).

In summary, Apostle MiniMax High Efficiency cfRNA Isolation Kit offers superior isolation efficiency of cell-free RNAs between 17 nt to 1000 nt, without phenol or chloroform. It is suitable for processing samples collected in various major blood collection tubes, especially specialized cfRNA BCTs which will prevent RNA degradation during storage.


Exhibit 1. Over 90% RNA recovery in range between 17 – 1000 nt.

RNA (100-1000 nt) and small RNA ladder (17-150 nt) was spiked in serum or plasma, followed by isolation with Apostle MiniMax High Efficiency cfRNA Isolation Kit (red curve), and compared with original RNA ladder (blue curve) using Bioanalyzer 2100 (A) RNA pico kit for 100-1000 nt and (B) small RNA kit for 17-150 nt fragments. Apostle MiniMax High Efficiency cfRNA Isolation Kit offers superior RNA and small RNA recovery efficiency of > 90%.

Exhibit 2. 1.1 to 2000 times higher exogenous RNA recovery rate than a column-based major alternative product.

cfRNA was isolated from plasma using Apostle MiniMax TM High Efficiency cfRNA Isolation Kit (red) or a column-based major competitor (green). A synthetic RNA mimic cel-miR-39 was spiked in during the extraction procedure as the reference to evaluate recovery rate. The qPCR amplification plot and CT value show 1.1, 9, and 2000 times higher RNA recovery rate when compared to column-based major alternative product for plasma collected in K3EDTA tube, cfRNA BCT vendor 1, or cfRNA BCT vendor 2, respectively.

Exhibit 3. 1.1 to 2.4 times higher RNA recovery rate than a column-based major alternative product.

cfRNA was isolated from plasma (collected in K3EDTA BCT) using Apostle MiniMax High Efficiency cfRNA Isolation Kit (red) or a major alternative product (green). RNA and small RNA targets including beta-globin, miR-21, U6, and miR-15 were quantified by qPCR amplification plots and CT values. The cfRNA amount isolated using Apostle MiniMax High Efficiency cfRNA Isolation Kit are 1.1 to 2.4 times higher than the column-based alternative product.

Exhibit 4. 3.5 to 480 times higher RNA recovery rate than a column-based major alternative product.

cfRNA was isolated from plasma (collected in cfRNA BCT vendor 1) using Apostle MiniMax High Efficiency cfRNA Isolation Kit (red) or a major alternative product (green). RNA and small RNA targets including beta-globin, miR-21, U6, and miR-15 were quantified by qPCR amplification plots and CT values. The amount of cfRNA isolated using Apostle MiniMax High Efficiency cfRNA Isolation Kit are 3.5 to 480 times higher than the column-based alternative product.

Exhibit 5. 1.6 to 6 times higher RNA recovery rate than a column-based major alternative product.

cfRNA was isolated from plasma (collected in cfRNA BCT vendor 2) using Apostle MiniMax High Efficiency cfRNA Isolation Kit (red) or a major alternative product (green). RNA and small RNA targets including beta-globin, miR-21, U6, and miR-15 were quantified by qPCR amplification plots and CT values. The amount of cfRNA isolated using Apostle MiniMax High Efficiency cfRNA Isolation Kit are 1.6 to 6 times higher than the column-based alternative product.

Nature 子刊 Nature Communications 近期发表又一项高影响力国际临床研究,其中应用 Apostle 艾铂图 MiniMax 游离核酸技术。此研究包括2125名癌症患者,9种不同的癌症类型。此研究表明,应用cfDNA游离核酸技术,可以有效、高敏感度地检测早期癌症。Apostle 艾铂图 MiniMax 游离核酸技术不断在国际上得到广泛的应用,迄今,已发表、被引用 Nature 子刊 3篇 ( Nature Medicine X 2, Nature Communications X 1), Science 子刊 1 篇, PNAS 1 篇,各种临床研究50余篇等。 祝贺此临床研究团队!期待Apostle 艾铂图技术更好地为人类做贡献。