Apostle MiniMax 高效游离 DNA 分离试剂盒 (S 版)

Powered by Apostle MiniMax technology, Apostle MiniMax High Efficiency cfDNA Isolation Kit (Type S) is an excellent tool for the isolation of ultra-low concentration cell free DNA (cfDNA). Apostle MiniMax High Efficiency cfDNA Isolation Kit (Type S) is featured for its efficient recovery of small DNA fragments ( <100 bp) from biological samples, where major alternative products fail. This feature is quite useful when small DNA molecules have significant presence in the biological sample and need to be isolated.

Compared to major alternative suppliers with magnetic bead technology, Apostle MiniMax High Efficiency cfDNA Isolation Kit (Type S) offers superior DNA isolation efficiency for cfDNA reference spiked in biological medium, especially for cfDNA with size <80 bp (Exhibit 1-3).

A) cfDNA reference standard (Horizon Discovery Ltd, Cat# HD780) was spiked in TE buffer, followed by isolation with Apostle MiniMax High Efficiency cfDNA Isolation Kit (Type S). The isolated DNA was characterized by Bioanalyzer 2100 (red curve), and compared with original cfDNA reference standard (blue curve). Apostle MiniMax High Efficiency cfDNA Isolation Kit (Type S) offers >95% recovery of the cfDNA reference standard, which has significant portion of DNA fragments with size <100 bp.

B) cfDNA reference standard was spiked in TE buffer, followed by isolation with MiniMax Type S and a major alternative product. Bioanalyzer 2100 analysis demonstrated significantly higher cfDNA recovery rate of MiniMax Type S (red curve) compared to a major alternative product (blue curve).

C) Zoom in of B）in the region between 35–100 bp. MiniMax Type S (red curve) is compared to a major alternative product (blue curve). Bioanalyzer 2100 analysis demonstrated the efficient recovery at ~50 bp of MiniMax Type S (red curve), while a major alternative product (blue curve) failed.